PSI-BLAST

or upload a local file
Select input mode
 single FASTA sequence   alignment  
Select alignment format
 
Select databases
Standard
(press CTRL to select/deselect multiple databases)
Genomes
 
Matrix
E-value
 
Perform ungapped alignment
 
Job-ID
 (leave empty for automatic creation)
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Altschul, S., Madden, T., Schaffer, A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl. Acids Res., 25 (17), 3389-3402.